Glial cell type-specific gene expression in the mouse cerebrum …
Mar 1, 2021 · Here we uncovered that the Gfa2 promoter is suitable for selective gene expression in astrocytes when used with the piggyBac system and in utero electroporation.
Sustained high level transgene expression in mammalian cells …
Mar 1, 2015 · The generated adenoviruses were shown to transduce mouse mesenchymal stem cells, iMEFs, with high efficiency (Fig. 1 B, panel b). Semi-quantitative RT-PCR analysis …
PiggyBac Toolbox - SpringerLink
Jan 1, 2012 · The PiggyBac (PB) transposon system was originally derived from the cabbage looper moth Trichoplusia ni and represents one of the most promising transposon systems to …
PiggyBac Mediated Multiplex Gene Transfer in Mouse Embryonic …
Dec 17, 2014 · PiggyBac system has been shown to have a high efficiency to mediate gene transfer. However, there are no reports on its efficiency to mediate multiplex transgenes in …
Oct 1, 2007 · The versatility of piggyBac in biotechnology applications for modification of the mammalian genome. piggyBac has been effective for mutagenesis in both cell lines and mice, …
Generation of transgene-free induced pluripotent mouse stem …
Mar 31, 2009 · piggyBac transposons carrying reprogramming factors are used to reprogram mouse embryonic fibroblasts, with efficiencies equivalent to retroviral transduction, and then …
PiggyBac Transposon-mediated Gene Transfer in Human Cells
Jan 1, 2007 · The piggyBac transposon system has been used for transgenesis of insects and for germline mutagenesis in mice. We compared transposition activity of piggyBac with Sleeping …
Manipulating piggyBac Transposon Chromosomal Integration Site …
Jul 5, 2011 · The piggyBac transposon system, derived from the cabbage looper moth Tricoplasma ni, is an emerging technology for efficient genetic modification of mammalian and …
Efficient Transposition of the piggyBac (PB) Transposon in Mammalian ...
Aug 12, 2005 · We have tested the ability of piggyBac (PB), a DNA transposon from the cabbage looper moth Trichoplusia ni, to transpose in mammalian systems. We show that PB elements …
A method of producing genetically manipulated mouse mammary …
The mammary reconstitution and transposon vector system to analyze gene function in vivo. a Schematic view of vector introduction and expansion of mammary gland.b Vector construct of …